Ambient mass spectrometry using desorption electrospray. Ambient ionization mass spectrometry today and tomorrow. Ions can be formed by extraction into charged electrospray droplets, thermally desorbed and ionized by chemical ionization, or laser desorbed or ablated and postionized before they enter the mass spectrometer. Extractive electrospray ionization is an spraytype, ambient ionization method that uses two merged sprays, one of which is generated by electrospray laserbased electrospraybased ambient ionization is a twostep process in which a pulsed laser is used to desorb or ablate material from a sample and the plume of material interacts with an electrospray to create ions. Ambient ionization and miniature mass spectrometry. The combination of ambient ionization and miniature mass spectrometry systems could most likely fulfill a significant need in medical diagnostics, providing highly specific molecular information in real time for c linical and even pointofcare analysis. In its first embodiment koichi tanaka used a cobaltglycerol liquid matrix and subsequent applications included a graphiteglycerol liquid matrix as well as a solid surface of porous silicon. These technologies usually involve separate ionization and sampleintroduction events, allowing independent. Pdf ambient ionization mass spectrometry researchgate. Mass spectrometric techniques frequently used in clinical diagnosis, such as gas chromatographymass spectrometry, liquid chromatographymass spectrometry, ambient ionization mass spectrometry, and matrixassisted laser desorption ionizationtimeofflight mass spectrometry malditofms, are discussed. However, detailed morphological information of the sample is often lost due to its limited spatial resolution. Ambient ms is a collection of techniques requiring no or minimal sample preparation, enabling analysis within seconds, and allowing for direct coupling to mass spectrometers with atmospheric pressure interfaces.
The resulting ions are drawn into a mass spectrometer for analysis. The difference between normal and ambient ms is mainly the pressure. Phthalate screening in food packaging by ambient ionization. The two main dims approaches for metabolomics and lipidomics are ms imaging and ambient ionization ms. Since the development of desorption electrospray ionization desi in 2004, many other ambient ionization techniques were developed. While commercially available ambient ionization mass spectrometry instrumentation in the specific configurations recommended by the committee in chapter 4 may not be currently available off the shelf, the major components have been commercialized and a number of. A nonpolar solvent with a dicationic ionic liquid was incorporated as the spray solution. The ambient ionization mass spectrometry methods keep the advantages of classical ms methods, that is, selectivity, sensitivity, informativity, performance and the possibility to analyze mixtures in the tandem mass spectrometry msms mode. While dozens of aims techniques have been introduced over the past two decades, their broad commercial and industrial use is still. Underlying principles of each technique are explained in detail, along with discussions on their applications across life science disciplines. Ambient ionization mass spectrometry ms allows direct. In desorption electrospray ionization desi, the principal method described here, electrically charged droplets are directed at the ambient object of. Ambient ionization mass spectrometry for chemical imaging. Direct analysis of dimethoate, tebuconazole, and trifloxystrobin on olive and vine leaves by desorption electrospray ionization interface, journal of mass spectrometry, 2017, 52, 11, 709wiley online library.
Selective and sensitive analysis by reactive easy ambient. Several ambient ionization methods have been created since 2004 and they utilize different mechanisms to. At the brigham and womens hospital at harvard medical school, for example, a mass spectrometer coupled to a desi source is currently being used in the surgical suite to acquire data from resected brain tissue. Gas chromatography mass spectrometry studies on aged. Electrospray, atmospheric pressure chemical ionization. While commercially available ambient ionization mass spectrometry instrumentation in the specific configurations recommended by the committee in chapter 4 may not be currently available off the shelf, the major components have been commercialized and a number of analytical instrument vendors are. Mass spectrometry sampling under ambient conditions with.
Ambient ionization and miniature mass spectrometry systems. The use of ambient ionization techniques such as dart 25, 26 and desi 2729, where chromatographic separation is not used, may further exacerbate the effects of. Recently, a new set of ambient ionization mass spectrometric ionization techniques have been introduced. Graham cooks, 1 zheng ouyang, zoltan takats,1,2 justin m. Ambient ionization and faims mass spectrometry for enhanced imaging of multiply charged molecular ions in biological tissues clara l. Blanksby1,4 1school of chemistry, university of wollongong, wollongong, nsw, 2522, australia, 2illawarra health and medical research institute ihmri, university of wollongong. Appendix c commercial sources of ambient ionization mass spectrometry instrumentation. In naims, a high voltage was applied between a tungsten nanotip and a metal plate causing a plasma to be generated which partially ionizes the sample. Written by acknowledged experts, there are full descriptions on how new ionization. Ambient ionization mass spectrometry for cancer diagnosis. Ambient mass spectrometry was introduced by cooks group in 2004 with the invention of desorption electrospray ionization desi.
Ambient ionization mass spectrometry for pointofcare diagnostics and other clinical measurements christina r. Analysis of polar components in crude oil by ambient mass. The combination of ambient ionization and miniature mass spectrometry systems could most likely fulfill a significant need in medical diagnostics, providing highly specific molecular information in real time for clinical and even pointofcare analysis. Optimization of ambient ionization methodologies for field investigations utilizing portable mass spectrometric instrumentation shahnaz mukta in the late nineteenth century, sir j. Ambient ionization mass spectrometry emerged as a new scientific discipline only about ten years ago. Ambient ionization mass spectrometry in life sciences. In this article, we describe ambient ionisation mass spectrometry techniques for the in situ analysis of intact proteins. Introduction in the field of biomedical analysis for clinical diagnosis and disease therapy, mass spectrometry ms has become a powerful tool due to its capability in the analysis of complex samples. In this study, we preliminary evaluated a selection of recent ambient ionization. Fifteen cocaine samples were analyzed, and all of them revealed positive tlceasims results for cocaine, but other drugs and adulterants were also detected such as lidocaine, caffeine, benzocaine. Principles and applications is a systematic introduction to this rapidly expanding area of study.
Mass spectrometric ionization methods that operate under ambient conditions and require minimal or no sample pretreatment have attracted much attention in such fields as biomedicine, food safety, antiterrorism, pharmaceuticals, and environmental pollution. Jan 01, 2016 ambient ionization mass spectrometry ms allows direct chemical analysis of unmodified and complex biological samples. There is a clinical need for new technolo gies that would enable rapid disease diagnosis based on. It was found that ions can be generated near a mass spectrometer at atmospheric pressure in open air under ambient conditions or directry in the field rather than. A reactive easy ambient sonicspray ionization method was developed for mass spectrometry analysis of hydrophobic analytes. C linical a pplication of am bi ent ion ization mass spectrometry vol. A method called nanotip ambient ionization mass spectrometry naims is described, which applies high voltage between a tungsten nanotip and a metal plate to generate a plasma in which ionized analytes on the surface of the metal plate are directed to the inlet and analyzed by a mass spectrometer. Nanotip ambient ionization mass spectrometry naims is designed to achieve both high resolution and simple operation in msi. Ambient ionization allows mass spectrometry analysis directly on the sample surface under atmospheric pressure with almost zero sample pretreatment. Underlying principles of each technique are explained in detail, along with discussions on their applications across life.
Thompsons experiment on cathode ray tubes led to the ultimate invention of the mass spectrometer. Recent advances in ambient mass spectrometry imaging. One driving motivation in the develop ment of pointofcare poc diagnostics is to conveniently. Ambient ionization has emerged as one of the hottest and fastest growing topics in mass spectrometry enabling sample analysis with minimal sample preparation. Ambient ionization and faims mass spectrometry for. Potential of recent ambient ionization techniques for future food. Potential of recent ambient ionization techniques for future. These technologies usually involve separate ionization and sampleintroduction events, allowing independent control over each set of. Surfaceassisted laser desorption ionization saldi is a soft laser desorption technique used for mass spectrometry analysis of biomolecules, polymers, and small organic molecules.
Potential of recent ambient ionization techniques for. A similar method, called laser desorption ionization mass spectrometry ldims 7, is another technique that is noninvasive and useful for the surface analysis. A considerable body of information has been reported since that time. Ambient ionization mass spectrometry marek domin, robert. Mass spectrometry has become a powerful tool in the field of biomedicine.
Wiseman a recent innovation in mass spectrometry is the ability to record mass spectra on ordinary samples, in their native environment, without sample preparation or preseparation by creating ions outside the instrument. Ambient ionization, miniature mass spectrometer, direct sampling, biofluid analysis, mass spectrometry imaging. Ambient ionization mass spectrometry ms allows direct chemical analysis of unmodified and complex biological samples. Ambient ionization has emerged as one of the hottest and fastest. This can be done using any types of mass spectrometers without essential redesign and one can obtain results in the real time mode with minimum sample preparation or. A rapid ambient ionizationmass spectrometry approach to monitoring the relative abundance of isomeric glycerophospholipids rachel l. Ambient ionisation mass spectrometry for in situ analysis. Ambient ionization allows mass spectrometry analysis directly on the sample surface under. Up to now, it has been successfully applied to analyze biological tissues 4, forensic samples 5, environmental samples 6 at atmospheric conditions without sample pretreatment. Ambient ionization methods permit the ionization of samples outside the mass spectrometer in the ordinary atmosphere, with minimal sample preparation. Pdf amb ient ionization mass spectrometry emerged as a new scientific discipline only about ten years ago. This suite of ionization techniques was introduced a decade ago and now includes a number of techniques, all seeking to minimize or eliminate sample preparation. Ambient ionization mass spectrometry aims has grown into a group of emerging analytical techniques that allow rapid, realtime, highthroughput, in situ, and in vivo analysis in many scientific fields including biomedicine, pharmaceuticals, and forensic sciences.
In desorption electrospray ionization desi, the principal method described here, electrically charged droplets are directed at the ambient object of interest. Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Ambient ionization mass spectrometry allows the rapid analysis of samples or objects in their native state in the open environment with no or little prior preparation. During the last decade, ambient ionization has emerged and quickly developed for direct analysis using mass spectrometry, with minimal sample preparation and. A rapid ambient ionizationmass spectrometry approach to. A lowcost, simplified platform of interchangeable, ambient.
Eberlin department of chemistry, the university of texas at austin, austin, texas 78712, united states s supporting information. Ambient ionization mass spectrometry for cancer diagnosis and surgical margin evaluation demian r. Jarmusch,1 valentina pirro,1 zheng ouyang,1,2 and r. Mass spectrometry imaging msi using ambient ionization technique enables a direct chemical investigation of biological samples with minimal sample pretreatment. Introducing the subject and explaining the basic concepts and terminology, this book will provide a comprehensive, unique treatise devoted to the subject. The introduction of ambient mass spectrometry aims could provide a solution for the previous mentioned problems. Because this technique is good at scanning the surface of a specimen, it achieves comprehensive twodimensional analysis like maldi imaging mass spectrometry 8, 9. Ambient ionization mass spectrometry rsc publishing. Galceran, applications in high resolution mass spectrometry, 2017, 265crossref. Clinical application of ambient ionization mass spectrometry jstage. Oct 15, 2004 this new method, termed desorption electrospray ionization desi, is related to other spray ionization methods, including electrospray ionization esi 2, 3, and to the desorption ionization methods, such as secondary ion mass spectrometry sims and laser desorption 4, 5. Ambient ionization and miniature mass spectrometry system for. Ambient surface mass spectrometry is an emerging field which shows great promise for the analysis of biomolecules directly from their biological substrate.
Clinical application of ambient ionization mass spectrometry. Ambient ionization mass spectrometry in life sciences 1st. Thanks to recent developments in ambient ionization and upcoming miniaturization of mass analyzers, transportable mass spectrometry may be added to the toolkit for onsite testing and eventually compete with multiplex immunoassays in mixture analysis. Zare department of chemistry, stanford university, stanford, california 94305, united states abstract. Sample preparation for probe electrospray ionization mass. Graham cooks at purdue university and now commercialized by prosolia, inc. Ambient ionization techniques combine a desorption process of the analytes, with their ionization. Optimization of ambient ionization methodologies for. The dependence of signal intensity is investigated as a function of the tipto.
Since the development of desorption electrospray ionization desi in 2004, many other ambient ionization. Request pdf on dec 1, 2012, ken smit and others published gas chromatography mass spectrometry studies on aged leafing grade aluminium flake find, read and cite all the research you need on. Dec 16, 2019 thanks to recent developments in ambient ionization and upcoming miniaturization of mass analyzers, transportable mass spectrometry may be added to the toolkit for onsite testing and eventually compete with multiplex immunoassays in mixture analysis. Due to their simplicity and low operation cost, rapid and onsite clinical mass spectrometry analysis becomes real. Pdf mass spectrometric ionization methods that operate under ambient conditions and require minimal or no sample pretreatment have attracted much. A new method of desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. These techniques allow desorption, ionization and characterization of analytes directly from surfaces or natural matrixes 6. In ambient ms, ionization of analytes is realized under regular pressures instead of high vacuum. Mass spectrometric ionization methods that operate under ambient conditions and require minimal or no sample pretreatment have attracted. Venter, ammonium bicarbonate addition improves the detection of proteins by desorption electrospray ionization mass spectrometry, journal of the american society for mass spectrometry, 2017, 28, 6, 1109crossref. The technique allows the analyses of a wide range of substances from various surfaces and matrices.
Recent advances in ambient mass spectrometry of trace. Ambient ionization mass spectrometry, which has witnessed a flurry of recent developments, is a set of useful techniques for the analysis of samples under openair conditions. Forensic applications of ambient ionization mass spectrometry. Surfaceassisted laser desorptionionization wikipedia. Forbes and edward sisco national institute of standards and technology, material measurement laboratory, gaithersburg, maryland, usa. Ambient ionization mass spectrometry for pointof care.
Will ambient ionization mass spectrometry become an. Ambient ionization is a form of ionization in which ions are formed in an ion source outside the mass spectrometer without sample preparation or separation. Ambient ionisation mass spectrometry for in situ analysis of. Will ambient ionization mass spectrometry become an integral. Phthalate screening in food packaging by ambient ionization high resolution msms mass spectrometry catharina crone 1, markus kellmann, frans schoutsen 2, elizabeth crawford 3 1 thermo fisher scientific, bremen, germany. Nanotip ambient ionization mass spectrometry analytical. Summing up, ambient ionization mass spectrometry includes openair methods for operation in ambient conditions or in the field using soft ionization outside the mass spectrometer. These methods, known collectively as ambient ms methods, have become also attractive alterna. Ambient ionization mass spectrometry for chemical imaging and detection of inorganic and organic explosives, narcotics, and other forensically relevant analytes thomas p. Ambient ionization mass spectrometry versus conventional lcgcms. Protein analysis by ambient ionization mass spectrometry using trypsinimmobilized organosiloxane polymer surfaces maria t.
Ambient ionization an overview sciencedirect topics. It allows direct, rapid, realtime, and highthroughput analyses with little or no sample pretreatment. Protein analysis by ambient ionization mass spectrometry. Mar 17, 2006 a recent innovation in mass spectrometry is the ability to record mass spectra on ordinary samples, in their native environment, without sample preparation or preseparation by creating ions outside the instrument. Mass spectrometric ionization methods that operate under ambient conditions and require minimal or no sample pretreatment have attracted much attention in such fields as biomedicine, food safety.